Inhibitory mechanism of polypeptide extract from scorpion venom on sarcoma S180 by promoting autophagy / 中草药

ABSTRACT

Objective: To explore the inhibitory mechanism of polypeptide extract from scorpion venom (PESV) on sarcoma S180. Methods: Thirty mice were implanted with S180 cells and randomly divided into three groups control group, PESV group, and rapamycin (RAPA) group with 10 mice in each group. Then the tumor volume growth curve was drawn and the tumor inhibitory rate (IR) was calculated. The morphological changes of the tumor tissue were observed by HE staining. The protein expression levels of Beclin1, MAP1LC3A, and CD133 were detected using immunohistochemical assay. Western blotting was applied to detecting the expression of Beclin1, MAP1LC3A, and CD133 in tumor tissue of mice in each group. Results: The growth of sarcoma S180 transplanted tumor was inhibited more obviously in the PESV group and RAPA group than that in the control group. The IR in the PESV and RAPA groups were 17.9% and 25.0%, respectively (P 180, the mechanisms might be associated with promoting the expression of autophagic relative factors, Beclin1 and MAP1LC3A as well as inhibiting the expression of CD133.