Simultaneous determination of seven triterpenoid saponins in Ilex kudingcha with HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1052-1055, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-854255
ABSTRACT
Objective:
To establish an HPLC method for simultaneously determining latifoloside G, latifoloside H, kudinoside G, latifoloside C, kudinoside A, kudinoside E, and kudinoside D in Ilex kudingcha.Methods:
The separation was performed on a YMC C18 column (YMC-pack ODS-A, 250 mm × 4.6 mm, 5 μm) with water-acetonitrile as the mobile phase in a gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was 210 nm and the column temperature was 30℃.Results:
Seven triterpenoid saponins all have good separating degree, good linear range, and linearity (r > 0.999 5). The average sample recovery rates were 99.25%-100.8%, RSD < 2%.Conclusion:
The established method is rapid, accurate, and with has repeatability and stability, which could provides the scientific evidence for the quality control of I. kudingcha.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2015
Tipo de documento:
Artigo
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