Simultaneous determination of five flavonoids components in different parts of Callistephus chinensis by HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 428-431, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-854340
ABSTRACT
Objective:
To establish an HPLC method for the simultaneous determination of five flavonnid components in different parts of Callistephus chinensis.Methods:
The chromatographic separation was performed on an XUnion C18 column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile and water, at a flow rate of 1 mL/min, and detected at 210 nm.Results:
The calibration curve was linear within 2.5-200 mg/L for hyperoside, apigenin-7-O-β-D-glucopyranoside, luteolin, apigenin, and kaempferol, respectively, with the correlation r > 0.999 0. The extraction recoveries varied from 95% to 105%.Conclusion:
The method is accurate and selective, and can be used for the quality control of different parts of C. chinensis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2015
Tipo de documento:
Artigo
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