Nitrogeous chemical constituents from Tinosporae Radix / 中草药

ABSTRACT

Objective: To investigate the chemical constituents from Tinosporae Radix (the roots of Tinospora sagittata) and their antiproliferative effects in cancer cells. Methods: The compounds were isolated and purified by means of silica gel, ODS, and Sephadex LH-20 column chromatography. The structures were identified on the basis of physicochemical properties and spectroscopic analysis. The antiproliferative effects in HL-60 and MCF-7 cells were tested by Trypan blue and MTT methods, respectively. Results: Nine compounds were isolated and their structures were identified as tetrahydropalmatine (1), tetrahydrojatrorrhizine (2), jatrorrhizine (3), palmatine (4), neoechinulin A (5), echinuline (6), N-trans-feruloyltyramine (7), uracil (8), and triethylamine hydroiodide (9). Conclusion: Among them, compounds 5, 6, 8 and 9 are isolated from the plants of Tinospora Miers for the first time. Compounds 1, 3-5 and 7 show the moderate antiproliferative effects in HL-60 cells.