Determination of flavonoids in Aurantii Immaturus Fructus by multi-components with single marker / 中草药

ABSTRACT

Objective: To establish a method for simultaneously determination for the content of four flavones (narirutin, naringin, hesperidin, and neohespridin) in Aurantii Immaturus Fructus by single marker, which is feasible and accurate. Methods: Taking hesperidin as internal standard substance, to establish a method for quantitative analysis of multi-components with single marker. To determinate the content of narirutin, naringin, hesperidin, and neohespridin in Aurantii Immaturus Fructus. The contents in 10 batches of samples were determined by both external standard method and QAMS. The The scientificity and feasibility of the method were evaluated by comparison of the quantitative results between external standard method and QAMS. Results: The relative correction factor (RCF) was perfect. The results calculated with a single marker were consistent with the results by the external standard method. Conclusion: The method with a single marker is accurate and feasible to evaluate the quality of Aurantii Immaturus Fructus.