Separation and preparation of polyphenols from leaves of Olea europaea by preparative medium pressure liquid chromatography / 中草药

ABSTRACT

Objective: To study the material basis of the anti-diabetic effective fraction in the leaves of Olea europaea. Methods: Preparative medium pressure liquid chromatography (PMPLC) was used for the separation and purification of the compounds. Their structures were elucidated by spectral analyses. Results: The structures of the compounds were identified by various spectroscopic methods combined with the data from some published literatures. There were five compounds which were extracted from the anti-diabetic effective fraction in the leaves of O. europaea and identified as decaffeoylverbascoside (1), luteolin-7-O-β-D-glucoside (2), 6″-O-β-D-glucopyranosyl oleuropein (3), oleuroside (4), and oleuropein (5). Additionally, decaffeoylverbascoside is isolated from this plant for the first time and the purities of the five compounds were all above 90% by HPLC detection. Conclusion: This research provides technological support for the development of new drug based on the leaves of O. europaea.