Specific PCR identification of Fritillaria thunbergii / 中草药

ABSTRACT

Objective: To identify Fritillaria thunbergii using special primer by specific PCR. Methods: Gene footprint of F. thunbergii named ZB1 was screened from RAPD amplification. Reclaimed ZB1 gene was inserted into T-vector to be cloned and sequenced. One pair of specific primers P2/P3 were designed according to the ZB1 sequence, and applied in specific PCR reaction using genome DNA of F. thunbergii as template. Results: A specific band around 750 bp was detected in F. thunbergii, while nothing appeared in other varieties. Conclusion: The method is convenient, reproducible, and precise, with broad application prospects.