Analysis on DNA fingerprints of Sarcandra glabra and correlation with its quality using ISSR molecular markers / 中草药

ABSTRACT

Objective: To analyze the correlation of DNA fingerprints of Sarcandra glabra with different quality levels and its quality. Methods: Using ISSR-PCR, 100 ISSR primers (UBC801-UBC900) were screened, and 23 of them were polymorphic. The 23 ISSR primers were used to amplify 18 S. glabra samples from different habitats. Based on the 18 ISSR amplified bands, the data base of amplified bands fingerprint was established using Excel. Results: one hundred and ninty-eight bands were obtained. Each primer amplified eight bands on average. The number of polymorphic DNA bands was 184, and the polymorphic proportion of DNA bands was 92.9%. Seven sites in two primers (UBC811 and UBC825) screened from 23 polymorphic ISSR primers were in one group, and seven sites in three primers (UBC827, UBC834, and UBC842) were in another group. The DNA fingerprints of 18 S. glabra samples were established, and U844-6 bands were screened from 184 polymorphic bands to correlate with sample quality. Conclusion: ISSR molecular markers could identify the DNA fingerprints of 18 S. glabra samples, and screen one band that is related to the quality.