UPLC fingerprint of Schisandra chinensis / 中草药

ABSTRACT

Objective: To establish the UPLC fingerprint of Schisandra chinensis. Methods: The chromatographic fingerprint was obtained with Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) and gradient eluted with acetonitrile and water; The flow rate was 0.5 mL/min, and the column temperature was maintained at 30°C. The detection wavelength was set at 216 nm, and the injection volume was 2 μL. Results: The common mode of UPLC fingerprint of S. chinensis was set up for the first time. There were 21 common peaks in the fingerprints. Schisandrin, schisandrol B, angeloylgomisin H, schisantherin A, schisantherin B, deoxyschizandrin, γ-schisandrin, gomisin N, and schisandrin C were identified by comparing the retention time and their ultraviolet spectra. The similarities of 19 batches of S. chinensis were above 0.970. Conclusion: The method is fast and efficiency. It can be used for the quality evaluation of S. chinensis.