Inhibition of Caudatin on migration of glioma C6 cells in vitro by downregulating β-catenin/survivin expression / 中草药

ABSTRACT

Objective: To investigate the effects of Caudatin on cell proliferation and migration of human glioma cell line C6 and the potential mechanisms. Methods: Cell viability was evaluated by MTT assay. Cell cycle distribution was assessed by propidium iodide flow cytometry. Scarification test and Transwell assay were used to measure the cell migration. The effects of Caudatin on the expression of β-catenin, Survivin, CyclinD1, and Cdk4 were determined by Western blotting assay. Results: Caudatin inhibited the C6 cell viability in a dose dependent manner, and caused an accumulation of C6 cells in G1 phase, and Western blotting results suggested that Caudatin inhibited the expression of CyclinD1 and Cdk4. The migration ability of C6 cells was significantly blocked by Caudatin treatment. Caudatin could significantly down-regulate the expression of β-catenin and Survivin in C6 cells. Conclusion: Caudatin could inhibit the C6 cell migration in vitro with a dose dependent way, and the potential mechanism might be related to inhibiting the expression of β-catenin and Survivin in C6 cells.