Antihepatoma spectrum-effect relationship of extract from Zanthoxyli Radix / 中草药

ABSTRACT

Objective: To study the relationship between HPLC fingerprint and antihepatoma activity of the extract from Zanthoxyli Radix. Methods: Zanthoxyli Radix fingerprint was established and human hepatic carcinoma cell line HepG2 was chosen as efficacy model. The antihepatoma activity of the extracts in Zanthoxyli Radix of 33 batchs from different habitats was determined by MTT method. After integrated the area of characteristic peak with the antihepatoma activity of Zanthoxyli Radix, the hepatic carcinoma "spectrum-effect" relationship was established by step-back technique. Results: The HPLC-fingerprint of Zanthoxyli Radix was established, and the similarity of fingerprint had reached above 0.7. All data were done with the chromatographic fingerprint similarity evaluation system software, chosen out 24 fingerprint peaks. The spectrum-effect relationship on HepG2 was established and this relationship was significantly different. The results indicated that the multiple linear regression could predict the antihepatoma activity to HepG2 with statistical and practical significance. Conclusion: The spectrum-effect relationship on HepG2 has the good predictive and describing abilities to evaluate the quality and therapeutic activity of Zanthoxyli Radix. According to the spectrum-effect relationship equation, we can predict the sensibility of different tumor cells.