Simultaneous determination for contents of naringin, hesperidin, and neohesperidin in flower of Citrus changshan-huyou by RP-HPLC / 中草药

ABSTRACT

Objective: To establish a method for the simultaneous determination for the contents of naringin, hesperidin, and neohesperidin in the flower of Citrus changshanhuyou by RP-HPLC. Methods: The HPLC method was adopted with Agilent Zorbax SB-C18 (250 mm × 4.6 mm, 5 μm) column; The mobile phase was acetonitrile-0.2% aqueous phosphoric acid (1585) with the flow rate of 1.0 mL/min. The detection wavelength was 284 nm and column temperature was 40°C; The peak areas were quantified by external standard method. Results: According to the method, naringin linear was within 28.45-284.5 ng, the regression equation was Y = 1725.2X + 6.383 3 (r = 0.9998), and the average recovery rate was 98.83%. Hesperidin linear was within 18.09-180.93 ng, Y=1764.6 X+0.4167 (r = 0.9998) with the average recovery rate of 99.05%. Neohesperidin linear was within 85.86-858.55 ng, Y = 1914.7X + 15.892 (r = 0.9996), and the average recovery rate was 98.83%. According to the established method, the contents of sample, naringin, hesperidin, and neohesperidin were respectively determined. Conclusion: The method is simple, rapid, accurate, and reliable, which provides the reference for the quality control for the flower of C. changshanhuyou, and is suitable for the content determination.