Simultaneous determination of ten effective components in Tongsaimai Pellets by UPLC-MS/MS / 中草药

ABSTRACT

Objective: To establish a UPLC-MS/MS method for simultaneously determining ten components (chromogenic acid, liquiritin apioside, calycosin-7-glucoside, cynaroside, liquiritin, ferulic acid, isoliquiritoside, harpagoside, liquiritigenin, and cinnamic acid) in Tongsaimai Pellets. Methods: A method for the simultaneous determination of the ten components was established by UPLC-MS/MS. The analysis was performed on a Waters Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with the mixture of acetonitrile and 0.1% formic acid/water as mobile phase, and the gradient elution at a flow rate of 0.3 mL/min. The analytes were detected by tandem mass spectrometry with the electrospray ionization (ESI) source combined with multiple reaction monitoring (MRM) mode. Results: The linear relationships between the concentration and peak areas of the ten components were chromogenic acid Y = 294.09 X + 17624; liquiritin apioside Y = 19.296 X + 748.42; calycosin-7-glucoside Y = 670.8 X + 11121; cynaroside Y = 490.45 X + 2938.3; liquiritin Y = 33.489 X + 555; ferulic acid Y = 127.76 X + 1343.5; isoliquiritoside Y = 57.87 X + 804.81; harpagoside Y = 13.125 X-20.365; liquiritigenin Y = 29.057 X + 367.71; cinnamic acid Y = 72.867 X + 1301.5 (r > 0.9990); The precisions, repeatabilities, and stabilities of the method were satisfactory, and the average recoveries were between 96.00% and 104.67% with the relative standard deviations no more than 3%. Conclusion: The established method is specific, rapid, and accurate for the determination of the ten effective components in Tongsaimai Pellets.