Study on chemical constituents of Myristicae Semen / 中草药

ABSTRACT

Objective: To study the chemical constituents in the dry mature seeds of Myristica fragrans (Myristicae Semen). Methods: Compounds were isolated and purified from the ethyl acetate layer of 75% alcohol extract by means of the silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC isolation methods. Compound structures were identified by analyzing and comparing the spectral data with those for them in the reference. Results: Eighteen compounds were obtained from the ethyl acetate layer of 75% ethanol extract in Myristicae Semen. On the basis of spectral data and combined with the references, these compounds had been identifited as: vanilic acid (1), butein (2), (2R)-3-(3', 4', 5'-trimethoxyphenyl)-1, 2- propanediol (3), sulphuretin (4), 3-methoxy-4, 5-methylenedioxy-cinnamic acid (5), 7, 3', 4'-trihydroxyflavanone (6), 7-hydroxy- 4-benzopyrone (7), verrucosin (8), (+)-erythro-(7S, 8R)-Δ8'-7-hydroxy-3, 4, 3', 5'-tetramethoxy-8-O-4'-neolignan (9), (-)-erythro-(7R, 8S)-Δ8'-7- acetoxy-3, 4, 3', 5'-tetramethoxy-8-O-4'-neolignan (10), nectandrin B (11), (-)-(7S, 7'R, 8S, 8'R)-4, 4'-dihydroxy-3, 5, 3'-trimethoxy-7, 7'-epoxylignan (12), fragransin B3 (13), fragransin B1 (14), (-)-enantiomer (15), (-)-erythro-(7R, 8S)-Δ8'-7-hydroxy-3, 4, 5, 3', 5'-pentamethoxy-8-O-4'-neolignan (16), (+)-erythro-(7S, 8R)-Δ8'-7, 4-dihydroxy-3, 5, 3', 5'-tetramethoxy-8-O-4'-neolignan (17), and (+)-5-methoxydeydrodiisoeugenol (18). Conclusion: Compounds 2 and 4-7 are isolated from the plants of Myristica Gronov. for the first time. Compound 1 is isolated from this plant for the first time.