Preliminary study on serum pharmacochemistry of Zhizi Dahuang Decoction / 中草药

ABSTRACT

Objective: To study the blood components in Zhizi Dahuang Decoction (ZDD) and to further explore its pharmacodynamic material basis. Methods: UFLC fingerprints of serum in rats after ZDD treatment were established with serum pharmacochemistry method. The UFLC profiles of serum samples collected after the treatment of ZDD, ZDD without one of the component herbs and single crude herb were compared, and the transitional constituents to blood and their metabolites were analyzed. Results: Twenty-one constituents absorbed into rat blood were detected after oral administration of ZDD, 12 of which were metabolites. Other nine were prototype constituents, three of which were recognized as geniposide, aloeemodin, and rhein by comparing with reference substances, respectively. Conclusion: The constituents of ZDD absorbed into blood are preliminarily identified, which could lay the foundation for the illumination of its pharmacodynamic material basis.