Molecular recognition of galangin molecular imprinting polymer microsphere / 中草药

ABSTRACT

Objective: To investigate the specific molecular recognition ability for galangin molecular imprinting polymer (GMIP). Methods: GMIP was prepared through the method of thermal polymerization with galangin as template molecular, acrylamide (AM) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as cross-linker, and tetrahydrofuran, acetonitrile, and acetone as pore forming agents, respectively. The GMIP was characterized by infrared spectroscopy and scanning electron microscopy (SEM). In addition, the GMIP was investigated in equilibrium binding experiment to evaluate its adsorption property and selective recognition. Results: The Scathard model analysis showed that two kinds of binding sites existed in the GMIP. The dissociation constant (Kd) and apparent maximum binding constant (Qmax) were Kd1 = 0.961 mmol/L, Qmax1 = 19.79 μmol/g for high affinity binding sites and Kd2 = 0.101 mmol/L, Qmax2 = 51.09 μmol/g for low affinity binding sites, respectively. Conclusion: GMIP has the specific adsorption and recognition capabilities to the galangin molecules. It can be a novel material for the separation and purification of the active ingredients from natural products.