Study on enzymolysis process for Whitmania pigra / 中草药

ABSTRACT

Objective: To optimize the enzymolysis process for Whitmania pigra. Methods: In order to select an appropriate index for study on the enzymolysis process, the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) of enzymatic products were determined before and after intestinal absorption, respectively, using an isolated everted intestine model. The emzymolysis of W. pigra was carried out by pepsin, trypsin, and the complex enzyme of pepsin and trypsin. According to the selected indexes, the enzymolysis process was optimized under different conditions, such as the kind of enzyme, ratio of enzymes to substrates, time of enzymolysis, and temperature of enzymolysis. Results: TT was an appropriate index. Enzymatic products with high activity could be obtained only using trypsin. After optimization, the best enzymolysis conditions were as follows 10% trypsin was added in the solution of W. pigra, and the reaction was proceeded under 73℃ for 8 h. Conclusion: TT is a reliable and sensitive anticoagulation index for enzymolysis. The enzymolysis technology is simple and stable, which could supply the supports for reasonable extraction of W. pigra.