Protection of terpenes and flavonoids from Chrysanthemi Indici Flos on immunological liver injury / 中草药

ABSTRACT

Objective: To study the protection of the terpenes and flavonoids from Chrysanthemi Indici Flos (CIF) on immunological liver injury (ILI) induced by concanavalin A (Con A) in mice. Methods: Mice were divided into control, model, CIF-A (terpenes), CIF-B (terpenes and flavonoids), and CIF-C (flavonoids in high-, mid-, and low-dose) groups, and with Bifendate Pills as positive control group. Mice were ig administered with the three extracts from CIF each once in the morning and afternoon of the first day and the next afternoon, then once daily for consecutive 7 d. The model of ILI was prepared by iv injection of Con A in the tail of mice in each group except the control group. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), inferon-α (TNF-α), and interferon-γ (IFN-γ) in serum of mice were analyzed, the level of MDA in liver was measured, and the histopathological changes of hepatic tissue were observerd. Results: Compared with the model group, CIF-A and CIF-B could obviously inhibit the increased levels of AST, ALT, and TNF-α in serum, while CIF-C could mainly reduce the levels of AST, ALT, IFN-γ, and CIF-B could decrease the level of MDA in the liver tissue with the significant amelioration of liver lesions. The effect of CIF-B was stronger than that of CIF-C and CIF-A. Conclusion: Terpenes and flavonoids from CIF have a certain protective effect on the liver of mice with ILI induced by Con A, and CIF-B has stronger effect.