Study on in situ intestinal absorption of Pulsatilla saponin D in rats / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3515-3520, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-854995
ABSTRACT
Objective:
To investigate the intestinal absorption characteristics of Pulsatilla saponin D in rats.Methods:
In situ single-pass intestinal perfusion model was used to inspect the absorption of Pulsatilla saponin D in the intestinal tract of rats. HPLC was used to determine the concentration of Pulsatilla saponin D in intestinal perfusion fluid samples. Absorption rate constant (Ka) and apparent permeability coefficient (Papp) were used as indexes to investigate the effects of absorption sites, drug concentration, different pH values, and P-glycoprotein (P-gp) inhibitor on Pulsatilla saponin D absorption.Results:
There was the significant difference (Pileum>jejunum>duodenum. With the pH value increasing, the Ka and Papp values also increased and both of them had significant differences (P0.05); There was significant difference (P<0.05) in Ka and Papp values with and without P-gp inhibitor.Conclusion:
Pulsatilla saponin D could be well absorbed in whole intestinal segments of rats, and the best intestinal absorption site is colon; The drug concentration in a certain range has no effect on Ka and Papp values, which preliminarily comfirms that the obsorption mechanism of Pulsatilla saponin D could be passive diffusion; Pulsatilla saponin D may be a substrate of P-gp and possess the saturation phenomenon of transporters.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2013
Tipo de documento:
Artigo
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