Establishment of cell suspension culture system and its alkaloid accumulation of Lycoris radiate / 中草药

ABSTRACT

Objective: To investigate the culture conditions for the establishment of cell suspension culture system for Lycoris radiata callus and its alkaloid accumulation. Methods: Using the bulbs of L. radiata as explants for callus induction, calli with good quality were selected for suspension cultivation. The factors influencing the establishment of L. radiata cell suspension culture system, such as hormone, inoculum amount, inositol, and conditional culture, were investigated. The alkaloid content in cell suspension culture system of L. radiata was determined by HPLC. Results: The optimal medium for the cell suspension culture was MS+0.5 mg/L 2, 4-D+0.1 mg/L KT+300 mg/L inositol and the inoculum size was 15%. The lag phase of the cell suspension culture system could be evidently shortened by the medium of logarithmic growth. The galanthamine contents in the cell suspension culture system and the medium were 4.18 and 0.69 times of those in the bulbs of L. radiata, respectively. Conclusion: The cell suspension culture system of L. radiata is established and it is a feasible way to gain galanthamine.