Chemical constituents in Akebiae Fructus based on UFLC-Q-TOF/MS / 中草药
Chinese Traditional and Herbal Drugs
; (24): 3282-3288, 2013.
Article
em Zh
| WPRIM
| ID: wpr-855009
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To identify the chemical constituents in Akebiae Fructus by UFLC-Q-TOF/MS method. Methods: The separation was performed on an Acquity UPLC BEH C18 Column (100 mm × 2.1 mm, 1.7 μm), with a mobile phase using 0.1% formic acid-acetonitrile and water containing 0.1% formic acid (B) for gradient elution. The flow rate was 0.3 mL/min, the temperature of column was 40°C with injection volume of 1 μL. TOF/MS and electrospray ion (ESI) source were applied for the qualitative analysis under the negative ion mode, and the full mass scan range was m/z 100-1500. Results: According to MS principle, twenty-five triterpenoids were identified from the methanol extract of Akebiae Fructus and the chemical structures of other nine unknown compounds were deduced. Conclusion: UFLC-Q-TOF/MS method could identify the main chemical constituents in Akebiae Fructus rapidly and accurately, which lays a foundation for the quality control of Akebiae Fructus.
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1
Índice:
WPRIM
Tipo de estudo:
Qualitative_research
Idioma:
Zh
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2013
Tipo de documento:
Article