Comparison on main growth characters of test-tube plants and seeding plants in Bupleurum chinense / 中草药

ABSTRACT

Objective: Analyzing hereditary parameters and comparing the test-tube plants population of Bupleurum chinense and seeding plants in different growing periods to provide the basis for building and screening out the right way to foster superior seeds of B. chinense. Methods: The test-tube plants were transplanted into the croplands and the same type of seeds were sowed at the same time. Until the next year, after the reviving of the plants, the plants were marked and the timing measure on the data of number of tiller, number of branches, number of leaves, length of leaves, width of leaves, number of jointings, length of jointing, sum of stem thickness, plant height, and number of flowers was carried out. Then, the roots were dug up and the medical characters of the roots, for instance, length, degree of thickness, and weight of the roots were measured. Following that, the statistic analysis on the data was conducted by using the software of SPSS 20.0. Results: The relative standard deviation values of test-tube plants population of B. chinense in diverse genetic characters were all relatively small. Moreover, plants were tidy, individual difference was tiny, and genetic characters were stable. In addition, the relevance of the characters of test-tube plants in various growing periods had some differences with the seeding plants. At last, compared with the seeding plants, the test-tube plants populations of B. chinense had more obvious superiority on the yield of roots and output of seeds. Additionally, the medicinal characters and quality were quite even. Conclusion: Compared with the seeding plants, the test-tube plants population of B. chinense has the superior botanical and pharmacognosy characters. Moreover, plant tissue culture technique is an effective way to breed and enlarge the superior seeds of B. chinense.