Study on brain drug concentration of geniposide in mice after iv injected with Xingnaojing Injection and its pharmacokinetics / 中草药

ABSTRACT

Objective: To develop an HPLC method for the determination of geniposide concentration in mouse brain and to investigate the pharmacokinetics after iv injection of Xingnaojing Injection. Methods: Forty mice were iv injected with Xingnaojing Injection 18 mg/kg (by geniposide), and the brain samples were collected at 1, 3, 5, 10, 30, 60, 90, 120 min after eyeball bleeding and 5 mice were sacrificed by cervical dislocation. The whole brain of mice was separated quickly to prepare brain homogenates, and the concentration of geniposide was detected by HPLC. The pharmacokinetic parameters were calculated by the software of Kinetica and the fitting method was noncompartmental. Results: The calibration curve was in good linear in the range of 54-1620 ng/g, r=0.9991. The extraction recoveries of geniposide brain drug concentration at 216, 864, and 1620 ng/g were (102.60 ± 4.28)%, (102.16 ± 4.48)%, and (97.66 ± 3.25)%, respectively. And the RSD values of inter-and intra-day were below 4.10%. The pharmacokinetic parameters were that the Cmax was (1 246.0 ± 520.7) ng/g, the tmax was 1 min, the MRT was (50.5 ± 1.9) min, and the AUC was (35780.3 ± 6148.0) ng/(g·min). Conclusion: The HPLC method for determining geniposide concentration in brain is simple, rapid, sensitive, and suitable for pharmacokinetic studies.