Isolation and purification of 5-hydroxytryptophan in extract from seeds of Griffonia simplicifolia by ion-exchange resin / 中草药

ABSTRACT

Objective: To research the adsortpion and separation function of ion-exchange resin on 5-hydroxytryptophan (5-HTP) in extract from the seeds of Griffonia simplicifolia and to screen the ion-exchange resin with high selective adsorption. Methods: The static adsorptive properties of eight different types of ion-exchange resins were evaluated using adsorptive content, desorption rate, adsorption rate, and adsorption temperature as indexes in order to optimize the resin. And the effects of different pH values, injection rates, injection concentration, eluent concentration, and elution rates on adsorptive properties of resins were investigated. Results: The 001 × 7 cation exchange resin showed the best comprehensive adsorption property. The loading solution concentration was 10.8 mg/mL, the pH value was 3.5, the flow rate of loading solution was 4.0 mL/min, and 3BV 7.0% ammonia water solution in 3.0 mL/min velocity was used to elute. In this process, the product was obtained with the 5-HTP purity of greater than 99.0% and the ash content of less than 1.0%, product average yield was 7.95% and the product quality could meet the market demand. Conclusion: The 001 × 7 cation exchange resin shows a better comprehensive adsorption property. It could be used to isolate and purify the 5-HTP for scale production.