Simultaneous determination of 7-hydroxy-4'-methoxyflavane and pterostilbene in Zhuang Medicine Dracaena cochinchinensis by HPLC / 中草药

ABSTRACT

Objective: To establish a method for the determination of 7-hydroxy-4'-methoxyflavane and pterostilbene in Zhuang Medicine Dracaena cochinchinensis. Methods: The determination was developed on C18 column. Acetonitrile-0.2% phosphoric acid (36: 64) was used as mobile phase and the detective wavelength was set at 281 and 306 nm, respectively. Results: The linear ranges of 7-hydroxy-4'-methoxyflavane and pterostilbene were 0.4076-1.5285 μg (r = 0.9998) and 1.6408-6.1530 μg (r = 0.9997), respectively. The average recovery was 97.88% (RSD = 0.82%) and 97.10% (RSD = 0.59%), respectively. Conclusion: The method is simple and accurate, which could be used for the quality control of D. cochinchinensis and its extract.