Study on preparation of natural medicine gum-bletilla scaffolds / 中草药

ABSTRACT

Objective: To explore the feasibility of natural constituents in Chinese materia medica used for the preparation of porous scaffold material as bone repair biomaterials and for the tissue regeneration and to study the preparation method and the properties of gum-bletilla scaffolds. Methods: Gum-bletilla was extracted from Bletilla Rhizoma, then purified, hydroformylated, mixed with other excipient, cross-linked, and lyophilized to get scaffolds. Results: High-purity standard gum-bletilla could be obtained by water extraction, alcohol-precipitation, and enzymatic purification. After oxidation, the gum-bletilla had Schiff base condensation to form reticular conformation, which was made into porous materials by freeze-drying. Conclusion: The scaffolds could be made of gum bletilla, which conforms to the requirements.