Comparison on HPLC fingerprint of Eucommiae Folium obtained from different habitats and by various original processing techniques / 中草药

ABSTRACT

Objective: To illustrate the influences of various original processing techniques on chemical composition of Eucommiae Folium from different habitats by comparing HPLC fingerprint profiles, so as to select the suitable techniques for processing Eucommiae Folium. Methods: To evaluate the quality of Eucommiae Folium with the various processing techniques by HPLC fingerprint. The analysis was performed on the Promosil C18(250 mm × 4.6 mm, 5 μm) column. Acetonitrile-0.1% H3PO4 was used as the mobile phase with gradient elution. The scanned wavelength was from 200 to 400 nm, and the detection wavelength was at 208 nm. The flow rate was set at 0.8 mL/min. The column temperature was set at 25°C. Results: The HPLC fingerprint profiles of Eucommiae Folium by the various processing techniques which were systematically researched were investigated. A total of main 22 common peaks were found, but the areas of the peaks were significantly different. The contents of chlorogenic acid and geniposidic acid obtained by the various processing techniques had the evident differences. Conclusion: HPLC fingerprint technique with the application to the content determination of the active ingredients has the good reproducibility and maneuverability. It is one of the potential process analytical techniques to explore the ingredient differences in the processed Eucommiae Folium from different habitats.