Simultaneous determination of six active constituent in different parts of Houttuyniae Herba by quantitative analysis of multi-components by single marker / 中草药

ABSTRACT

Objective: To establish a method for simultaneous determination of the contents of six main active constituents (neochlorogenic acid, chlorogenic acid, rutin, hyperin, isoquercitrin, and quercitrin) in Houttuyniae Herba by quantitative analysis of multi-components by single marker (QAMS). Methods: The chromatographic separation was achieved on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid aqueous with gradient elution and changeable wavelength detection. Chlorogenic acid was used as the reference, the calibration factor of other five constituents to that of chlorogenic acid was calculated respectively to get each relative calibration factor. And the contents of the active constituents were calculated by the relative calibration factor to realize QAMS. At the same time, the external standard method was used to detect the contents of the six constituents, comparing the two methods to validate the accuracy and feasibility of the new method. Results: No significant differences were found in the quantitative results of the six active constituents in Houttuyniae Herba determined by measured value and calculated value. The amounts of the six active constituents in the different parts of Houttuyniae Herba were found to be varied, chlorogenic acids and flavonoids mainly distributed in the leaves. Conclusion: The active constituents of Houttuyniae Herba mainly distribute in the leaves. The QAMS is feasible and accurate to evaluate the contents of the six active constituents in Houttuyniae Herba.