Rapid recognition and identification of phenanthrenes in Juncus effusus and Juncus setchuensis by LC-ESI-MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1539-1545, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-855271
ABSTRACT
Objective:
To rapidly recognize and identify phenanthrenes in Juncus effusus and J. setchuensis.Methods:
HPLC-ESI-MS method was used to investigate the ESI-MS characteristics of those phenanthrenes, to screen, and to identify phenanthrenes in J. effusus and J. setchuensis.Results:
Under negative mode, phenanthrenes were inclined to loss two hydrogens, which presumably caused by the cyclization of vinyl and aromatic ring; Furthermore, the loss of C=O could be also observed. Fragmentation pathway of phenanthrenes in ESI as a negative ion mode was summarized. Twenty-one phenanthrenes were rapidly detected from the extracts of J. effusus and J. setchuensis. Among them, six compounds were accurately identified by comparison with reference substances; The structures of five compounds were inferred according to their MS spectrum and polarity.Conclusion:
HPLC-ESI-MS provides not only a new technique for the rapid identification of phenanthrenes from complex matrix, but also an effective method for the target separation of constituents in Chinese materia medica.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2013
Tipo de documento:
Artigo
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