Chemical constituents from underground parts of Isodon sculponeatus and their bioactivities / 中草药

ABSTRACT

Objective: To study the chemical constituents from the underground parts from Isodon sculponeatus and their bioactivities. Methods: The compounds were isolated with 70% acetone by chromatography with silica gel, RP-18, and Sephadex LH-20 columns. The obtained parts were identified by means of spectroscopic methods. The cytotoxic activities of the samples including 70% acetone extract, ethyl acetate (EtOAc) fraction, and butanol fraction were evaluated by MTT method. Results: From the EtOAc fraction in the underground parts of I. sculponeata, fourteen compounds were isolated and identified as macrophynin E (1), 19-hydroxyferruginol (2), 2α, 3α, 24-trihydroxyursa-12, 20(30)-dien-28-oic acid (3), hyptadienic acid (4), stigmast-4-en-6β-ol-3-one (5), stigmastane-3β, 6α-diol (6), coniferyl aldehyde (7), 1, 2-benzenedicarboxylic acid bis (2-methylpropyl) ester (8), bis (2-ethylhexyl) phthalate (9), n-hexadecanioc acid (10), 5α, 8α-ergosta-6, 22E-dien-3β-ol (11), daucosterol (12), β-sitosterol (13), and ursolic acid (14). Results The bioassays indicated that both the 70% acetone extract and EtOAc fraction displayed the moderate cytotoxicities against the liver cancer cell line SMMC-7721. Conclusion: All the compounds are isolated from the underground parts of I. sculponeata for the first time while compounds 1-10 are firstly isolated from this plant, and compounds 2, 3, 5, 6, 8, and 9 are isolated from the plants of Isodon (Hara.) for the first time.