Anti-inflammatory activity of Weichang'an Pill / 中草药

ABSTRACT

Objective: To study the active constituents in the methanol extract of Weichang'an Pill (MEWP) and their anti-inflammatory activity. Methods: The HPLC method was used to anylyze the constituents in MEWP. The anti-inflammatory effects of MEWP were observed on the acute anti-inflammatory models of xylene-induced ear edema, carrageenan-induced paw edema, and acetic acid-induced vascular permeability; Meanwhile the chronic anti-inflammatory model of granuloma was induced by cotton pellet implantation. Results: Twelve active constituents in MEWP were analyzed, including costunolide and dehydro-α-curcumene from Aucklandiae Radix; naringin, hesperedin, and neohesperedin from Aurantii Fructus; magnolol and honokiol from Magnoliae Officinalis Cortex; aloe-emodin, rhein, emodin, chrysophanol, and physcion from Rhei. Radix et Rhizoma. At the dose of 800 mg/kg, the MEWP showed the inhibitory rate of 39.46% on the ear edema and 35.37% on the increasing of capillary permeability induced by acetic acid in mice. It could decrease the paw edema caused by carrageenan after 1 h and inhibit the granuloma induced by cotton pellet implantation. Conclusion: The MEWP could antagonize the reaction of inflammation.