Differences of metabolic profiling of Vladimiriae Radix before and after simmer processing by HPLC-UV technology / 中草药

ABSTRACT

Objective: To compare the metabolic profiling of Vladimiriae Radix (VR) before and after simmer processing and to screen out the characteristic constituents leading to some differences. Methods: The chromatogram data sets of the processed and unprocessed VR were obtained by HPLC-UV analysis; The metabolic profiling differences of VR before and after processing were compared by principal component analysis, partial least square-discriminant analysis, and hierarchical cluster analysis, then the characteristic constituents were screened out. Results: The metabolic profilings between processed and unprocessed VR were apparently different, and the data showed seven constituents among them mainly made the quality difference, including costunolide and dehydrocostus lactone. Conclusion: The processed and unprocessed VR have the significant differences on the metabolic profiling.