Effects of different physicochemical factors on growth of tissue culture seedlings and accumulation of total flavonoids in Saussurea involucrata / 中草药

ABSTRACT

Objective To investigate the effects of physicochemical factors on the growth and total flavonoids accumulation of Saussurea involucrata. Methods The dry weight and total flavonoids accumulation in multiple seedings of S. involucrata were measured. Results The optimal cultural condition for the growth and total flavonoid accumulation of S. involucrata was as following MS culture medium adding 6-BA 0.5 mg/L + NAA 0.05 mg/L, 40 mmol/L of nitrogen source, NH4+-NO3- (15 25), 40 g/L of carbon sucrose, pH values 5.0-6.0, illumination intensity of 2 500 Ix, temperature 25°C. Conclusion The optimal culture conditions could significantly improve the growth and total flavonoids accumulation of S. involucrata. And the nitrogen, carbon, and temperature have the most obvious effects on the growth and total flavonoids accumulation of S. involucrata.