Effects of growth regulator and culture methods on rooting of Ajuga lobata and content of β-ecdysone / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1828-1832, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-855545
ABSTRACT
Objective:
To study the effects of indole butyric acid (IBA) and NAA at different concentrations and culture methods on the growth of Ajuga lobata root and content of β-ecdysone.Methods:
Taking MS as basic medium supplemented with IBA (0.5, 1.0, and 2.0 mg/L) or NAA (0.3, 0.6, and 0.9 mg/L), the tissue culture was carried out. HPLC was used to determinate the content of β-ecdysterone.Results:
The results showed that the roots could grew well on MS as basic medium supplemented with IBA 0.5-1 mg/L. The best culture condition for the highest β-ecdysone accumulation was MS medium supplemented with IBA 2 mg/L. The content of β-ecdysterone in the whole plant and the roots of A. lobata with tissue culture was twice times of hydroponics and about three times of cultivation.Conclusion:
MS medium supplemented with IBA at the proper concentration could promote the root growth of A. lobata. The content of β-ecdysterone in the root of A. lobata is the highest in tissue cultures.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2011
Tipo de documento:
Artigo
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