Optimization of synthetic process of PEGylated puerarin by orthogonal test with multi-index / 中草药

ABSTRACT

Objective: To optimize the synthesis of PEGylated puerarin (PEG-PUE) by orthogonal test with multi-index evaluation. Methods: The research has focused on the optimized interacting factors of the synthetic process of PEG-PUE by HPLC, such as ratio of PEG and PUE, catalyst DMAP dosage, and reaction time, using the purity, drug loading, and yield of PEG-PUE as evaluation indexes. Results: The optimum synthesis conditions of PEG-PUE were as follows, PEG-EDC-PUE-DMAP (1 1.2 1.2 0.3) and reaction time 12 h. Conclusion: The optimum synthesis condition of PEG-PUE is reasonable, as well as feasible.