Bio-evaluation methods and optimization for Isatidis Radix quality control based on antiviral activity detection / 中草药

ABSTRACT

Objective: To establish and optimize a bio-evaluation method based on antiviral activity detection for Isatidis Radix quality control. Methods: According to the fact that antivirus activity is the main pharmacological effect of Isatidis Radix, the means of hemagglutination activity detection and the means of influenza virus neuraminidase (NA) activity detection were used for establishing two antiviral activity detection methods for the bio-evaluation of Isatidis Radix. In addition, contrastive analyzation and optimization of the two methods were carried out in this study. Results: The two methods could be used to evaluate and distinct the biological activities of different batches of Isatidis Radix samples and both methods have good repeatabilities (RSD = 7.0% and 5.78%). The result shows a good correlation (P<0.01, r = -0.81) between the neuraminidase inhibitory activity and the hemagglutination activity of Isatidis Radix. The relationship between agglutination activity and antiviral effect of Isatidis Radix was further verified. Agglutination detection method has the superiority to its safety, inexpensiveness, easiness, and practicality. Conclusion: This novel method could reflect the pharmacodynamic activity of Isatidis Radix on anti-influenza virus and be helpful for the quality control of Isatidis Radix. This study provides reference for the bio-evaluation on the quality control of Chinese materia medica.