Identification of homoisoflavonoids in Ophiopogon japonicus alcohol extract by an LC-MS based on precise mass and tandem mass spectrometer / 中草药

ABSTRACT

Objective: To develop an LC/MS approach based on precise mass and tandem mass spectrometer to identify homoisoflavonoids from Ophiopogon japonicus alcohol extract. Methods: Separation was performed on an Alltima C18 reverse-phase column (250 mm x 4.6 mm, 5 μm) and an Alltech pre-column. The mobile phase consisted of water containing 1 mmol/L ammonium acetate-acetonitrile containing 0.01% acetic acid was used as gradient elution. The flow rate was 1.0 mL/min. The detection wavelength was 298 nm. A combination of time-of-flight mass spectrometer (TOF/MS) and ion trap tandem mass spectrometer (Trap/MSn) was applied for qualitative analysis under negative ion mode. Results: From the alcohol extract of O. japonicus, the molecular formulae of 11 components were screened by TOF/MS and 11 chemical components were identified by Trap/MSn. All of the peaks were attributed. Conclusion: This method can be used to identify medicinal ingredients quickly and provide an effective and reliable analytical model, as well as other complex system of traditional Chinese medicine compound.