Determinaton of five flavonoids from different processing products of Artemisia frigida by HPCE / 中草药

ABSTRACT

Objective: Determining the content of five flavonoids in Artemisia frigida by high performance capillary electrophoresis (HPCE) method to provide reference data for the evaluation of processing products of A. frigida. Methods: Treated by different processing technologies, the content of five flavonoids compounds can be determined and compared by HPCE. Results: Regression equations were 5,7,3′-triterhydroxy-4′-methoxyflavone Y = 0.114 3 X + 0.032 7, r = 0.999 8; 5,3′-dihydroxy-6,7,4′-tritermethoxyflavone Y = 0.100 9 X + 0.048 5, r = 0.999 6; quercetin Y = 0.055 5 X + 0.026 8, r = 0.999 6; 5,7,3′-triterhydroxy-6,4′-dimethoxyflavone Y= 0.113 2X-0.016 1, r = 0.999 3; luteolin Y = 0.097 9X-0.029 5, r = 0.999 4. Five kinds of flavonoids were good linear relationship at 1.00-40.00, 10.00-200.00, 5.00-100.00, 1.00-40.00, 1.00-40.00 μg/mL. The average recoveries were 98.44%, 97.75%, 97.73%, 97.98%, and 98.07%. And RSD were 1.60%, 1.03%, 1.57%, 0.94%, and 1.20%. Conclusion: The results reveal that the different processing technologies have different effects on the content determination of five flavonoids compounds in A. frigida. Using the five flavonoids as testing indexes, the best processing technology of A. frigida is heating and drying to constant weight at 60 °C.