Multicolor fluorescence in situ hybridization analysis on rDNA and telomere of Isatis indigotica / 中草药

ABSTRACT

Objective: To investigate the physical location of 45S, 5S rDNA, and telomere sequences and analyze the karyotype of the metaphase chromosomes of Isatis indigotica. Methods: Multicolor fluorescence in situ hybridization (FISH) approach was used to detect the physical location of the above sequences. Results: One pair of 45S rDNA sites located on chromosome 4, one pair of 5S rDNA sites located on chromosome 5 and 14 pairs of telomere sites located at the ends of each chromosome in I. indigotica were detected. The karyotype formula is 2n = 2x = 14 = 10m (2SAT) + 4sm, which belongs to 2A karyotype. Conclusion: This research provides effective cytological markers for karyotype analysis and construction of a molecular cytogenetic map of I. indigotica.