Biotransformation optimization of swertiamarin by Aspergillus niger / 中草药

ABSTRACT

Objective: With swertiamarin as the substrate, biotransformation conditions of swertiamarin were optimized by Aspergillus niger. Methods: With productivity ratio of EHPO {(2)-5-ethylidene-8-hydroxy-3,4,5,6,7,8-hexahydro-1H-pyrano [3,4-c] pyridine-1-one} and transformation ratio of swertiamarin as indexes, training time, carbon resource, nitrogen resource, metal ions, phosphate, growth factor (yeast extract paste), inoculum size, substrate concentration, initial pH value, and tempuraure were evaluated to optimize the biotransformation of swertiamarin. Results: It confirmed the optimization cultivating condition that glucose concentration was 10 g/L, peptone concentration was 5 g/L, yeast extract paste was 5 g/L, KH2PO4 concentration was 5 g/L, MgSO4 and CaCl2 concentration was 1 g/L, initial pH value was 6.0, inoculum size was 0.5%, substrate concentration was 1 mg/mL, cultivation temperature was 28 °C, and cultivating time was 5 d. Conclusion: The biotransformation ratio for EHPO into swertiamarin biotransformed by A. niger could steadily be about 8%.