Construction of tissue microarrays in cerebral ischemic rats / 中国脑血管病杂志

ABSTRACT

Objective: To construct tissue microarrys in rats with ischemic brain injury and to provide an experimental means for the study of ischemic brain injury. Methods: Fifty male Sprague-Dawley rats were randomly assigned to cerebral ischemia 1-, 4-, 7-, 14- and 28-day groups, and each group had 8 rats. Sham-operation group (2 rats) was established at each time points. The model of global four-vessel occlusion was used. Cortex of temporal lobe, hippocampus and corpus callosum were selected as observing points. The tissue microarrys in ischemic brain injury rats were constructed in the order of designing microarrys, prefabricating recipient paraffin blocks, localizing donator paraffin blocks, sampling, slicing continuously and baking slice, etc. Results: One hundred and fifty lattice arrays with 15 × 10 were constructed on 36 min × 26 mm × 15 mm recipient paraffin blocks, and 150 tissue specimens were distributed, each lattice was 1.0 mm in diameter, and the microarrys were cut into 4 μm per layer. The lattice lattices distributed evenly under macroscopic observation, there were no fissuarings on paraffin blocks, and the point lattices did not fall off on microscope slide. Six point lattices displaced, and the displacement rate was 4%. HE stain showed sharp margin surrounding sampling tissues without obvious tissue crushing and deformation. Conclusion: The successful construction of tissue microarry may provide a convenient and efficient experimental means for the study of ischemic brain injury.