Anesthesia selection in the establishment of the model of middle cerebral artery occlusion in rats / 中国脑血管病杂志

ABSTRACT

Objective: To study the effect of intraperitoneal injection anesthesia and endotracheal anesthesia during creating a rat model of middle cerebral artery occlusion (MCAO). Methods: Rats were anesthetized and maintained respectively with chloral hydrate by intraperitoneal injection and inbreathing 2% halothane and 30% O2 via tracheal intubation. MCAO model was created by method of Zea Longa. The time of induction, maintenance, recovery and physiological indexes were monitored, and the result of blood gas analyses, blood glucose, brain temperatures, rectal temperatures, neurological deficit, 48 hours' survival rate and the volume of brain infarction were also studied. Results: Intraperitoneal injection anesthesia inhibited the respiration significantly. Blood gas analysis indicated serious respiratory acidosis (pH 7.29 ± 0.03, PCO2 56.8 ± 4.2 mm Hg, PO2 119.1 ± 15.6 mm Hg). But it appeared approximately normal in rats with halothane anesthesia (pH 7.48 ± 0.06, PCO2 36.2 ± 0.3 mm Hg, PO2 219.1 ± 27.4 mm Hg). There was significant difference between two kinds of anesthesia in pH, PCO2, PO2 (all P 0.05). There was no significant difference of survival rate at 48 hours and brain infract volume between the two kinds of anesthesia (P > 0.05). Conclusion: This study suggestes that endotracheal anesthesia with 2% halothane and 30% O2 is better than intraperitoneal injection of chloral hydrate. It is effective and less influence on the brain temperature and other relative indexes during creating middle cerebral artery ocelusion rat model.