Study on distribution of succinylcholine chloride at poisoning dose in rats by UHPLC-MS/MS analysis / 中国药理学通报

ABSTRACT

Aim To determine the distribution of succinylcholine chloride (Suc) at toxic dose in rats by establishing UHPLC-MS/MS analysis. Methods The rats were subcutaneously injected with three doses of Suc, and 4 h later, rat serum and major organs were taken for acetonitrile protein precipitation and purified by solid phase extraction with WCX column. Luna NH2 column (2 mm × 100 mm, 3 μm) was used for chromatography. The mobile phase was 0. 1% formic acid-acetonitrile isoelution. Mass spectrometry was carried outwith positive ion scanning multi-reaction monitoring mode (MRM). Qualitative and quantitative analysis was conducted on the ion pairs of Suc (m/z 145. 1→93.3, m/z 145.1→115.4). Results The detection limit of Suc was 0.01 μg · L-1, the recovery rate was 89. 5% ∼ 95. 4%, and the intraday and mtraday RSD was less than 15%. Except in the untreated rat, Suc was detected in the serum, heart, liver, kidney and other major organs from Sue-administered rats, and Suc content in the kidney was the highest among organs. Conclusions Sucat poisoning dose is distributed in the serum, heart, liver, kidney and other tissues. The content in the kidney is the highest; UHPLC-MS/ MS analysis is an effective and reliable method for detecting the drug content in Sue-poisoned rats.