Effect of catalpol on ages-stimulated raw264.7 macrophage mediated mouse mesangial cell injury / 中国药理学通报

ABSTRACT

Aim To investigate the effect of catalpol on the stimulation of macrophage-mediated mouse mesang-ial cells ( MMCs) injury by advanced glycation end products (AGEs). Methods MMCs and RAW264.7 macrophages were co-cultured in vitro and divided into control group, model group,catalpol group (0. 1, 1.0, 10. 0 jjimol • L"1 ) , and aminoguanidine group ( 10. 0 fimol • L"1) , which was setas the positive control. After drug administration for 1 h, RAW264. 7 was stimulated by AGEs (100 mg • L_l) for 24 h. MTT assay was employed to detect the proliferation rate of MMCs . Immunofluorescence was used to investigate the expres-sion levels of COL-IV in MMCs. Western blot was applied to assess the expression of FN,COL-IV and TGF-P in MMCs. ELISA was utilized to determine the levels of IL-6, IL-12 and TNF-ot in supernatant liquid of RAW264. 7 macrophages. Results Catalpol could inhibit RAW264.7 macrophage-mediated MMC-induced proliferation stimulated by AGEs ( P < 0. 05, P < 0.01), down-regulate the levels of FN, COL-IV and TGF-0 proteins in MMCs (P < 0. 05, P < 0. 01) , and decrease IL-6, IL-12 and TNF-a level (P < 0. 05, P < 0. 01). Conclusions Catalpol has apparent protective effect on AGEs-stimulated macrophage-mediated me-sangial cell injury. It down-regulates FN, COL-IV and TGF-p protein expression in MMCs, down-regulates inflammatory factor levels and reduces inflammation, thereby alleviating diabetic kidney damage.