Effect of astragalus polysaccharide on autophagy and PI3K/AKT signaling pathway in lung cancer A549 cells induced by xanthine oxidase / 中国药理学通报

ABSTRACT

Aim To observe the regulatory effects of astragalus polysaccharide (APS) on AKT/PI3K signa-ling pathway in lung cancer A549 cells. Methods The autophagy model was established by xanthine oxidase (XOD). Then the intervention was carried out with 100, 200, 400 mg • L"1 of APS and 3-methylade-nine (3-MA). Cell Counting Kit-8(CCK-8) method, transmission electron microscope and Western blot were used to detect the effect of APS on the proliferation, autophagy and autophagy marker molecules respectively in lung cancer A549 autophagy model. Results Compared with blank group, cell growth of the model group was accelerated; the number of autophagosomes increased , and the expressions of microtubule-associated protein 1 light chain-3B (LC3B) and PI3K significantly increased, while p62 and AKT markedly decreased (P < 0. 05) . Compared with model group, cell growth slowed down, and the number of autophagosomes decreased; the expression of LC3B and PI3K significantly decreased. However, p62 AND AKT expression significantly increased (P <0. 05) in 200 or 400 mg • L"'of APS-treatedgroups. Compared with 3-MA-treated group,the expression of LC3B significantly decreased in 200 mg • L"1 of APS-treated group(P <0. 05), and PI3K significantly decreased in 400 mg • L"1 of APS-treated group (P < 0. 05). Conclusion APS may exert anti-tumor effect by down-regulating PI3K/AKT signaling pathway of human lung cancer A549 cells.