Effects of coenzyme Qi0 on bone microstructure and myofibril structure induced by D-galactose in male mice / 中国药理学通报

ABSTRACT

; Aim To observe the effects of CoQ10 on bone microstructure and myofibril structure induced by D-galactose in male mice. Methods Forty male mice were randomly divided into control group, model group, calcitriol group and CoQ10 group, in which drugs were given for 12 weeks. At the end of the experiment, the mice were sacrificed and the femurs were taken for micro-CT and biomechanics analysis. The gastrocnemius muscle was taken for transmission electron microscope examination. Results Compared with control group, the maximum load and stiffness of femur in model group significantly decreased (P < 0. 01). The micro-CT parameters showed that Conn. D and BMD markedly decreased (P < 0. 05), while Tb. Sp evidently increased (P <0. 05). In model group, the gastrocnemius muscle fibrils parameters showed that the length of sarcomere, I-band, H-zone, M-line and Z-line significantly increased (P < 0. 01), while the length of overlapped actin and myosin filaments significantly decreased (P < 0. 01) . Compared with model group, the maximum load, Conn. D., BMD and Tb. Th of femur in coenzyme Q 1 0 group markedly increased (P < 0. 05), while SMI and Tb. Sp significantly decreased (P < 0 . 01) . Moreover, I-band, H-zone, M-line and Z-line were significantly shortened (P < 0 . 01) . Conclusion Coenzyme Q10 can improve bone microstructure damage and the contractility of gastrocnemius muscle in male mice induced by D-galactose.