Role of autophagy in IL1 ßinduced apoptosis of rat pancreatic INS1 islet β cells / 中国药理学通报

ABSTRACT

Aim To investigate the role of autophagy in IL1 ßinduced apoptosis of islet ß cells. Methods Rat ß cell line INS1 was cultured in vitro. The survival rate of INS1 cells was measured by CCK8 method at different concentrations and time. The level of apoptosis was analyzed by flow cytometry and Western blot, and the expressions of autophagyrelated proteins were detected by Western blot. Results ILlβ could reduce the survival rate of INS1 cells in a concentrationand timedependent manner (P < 0.05, P < 0. 01). Compared with control group, with the increase of IL1 β concentration, the level of apoptosis of INS1 cells increased gradually (P < 0. 05); at the same time, the expression of autophagy increased gradually (P < 0. 05). Pretreatment with autophagy agonist rapamycin (RAPA) could increase autophagy (P < 0. 05) and apoptotic level (P < 0. 05). However, the expression of autophagy and apoptosis decreased after pretreatment with autophagy inhibitor 3MA (P < 0 . 0 5) . Conclusion IL1 ß can promote the apoptosis of INS1 cells by inducing autophagy.