Effects of Lidamycin on cell cycle, apoptosis, autophagy and epithelial mesenchymal transformation of human cervical carcinoma Hela cells / 中国药理学通报

ABSTRACT

Aim To investigate the effects of lidamycin (LDM) on cell cycle, apoptosis, autophagy and epithelial mesenchymal transformation (EMT) of human cervical cancer Hela cells as well as its underlying mechanism. Methods MTT assay was used to analyze the effect of LDM on Hela cell growth. Flow cytometry was used to detect the effect of LDM on cell cycle, apoptosis and mitochondrial membrane potential. Western blot was used to detect the expression levels of proteins. Transwell assay was used to analyze the effect of LDM on migration and invasion of Hela cells. QT-PCR was employed to analyze the effects of LDM on LSD1 (lysine specific demethylase 1) expression level in Hela cells. Results LDM could significantly inhibit Hela cell proliferation in a time-and concentration-dependent manner, with the IC50 values of 19. 24 ng . mL-1 (24 h), 6.678 ng . mL- 1(48 h) and 3.221 ng . mL-1 (72 h). LDM could induce G2/M cycle arrest and mitochondrial-mediated endogenous apoptosis in Hela cells. Low concentration of LDM(5 ng . mL-1) could induce autophagy, but high concentration of LDM(20 ng . mL-1 ) could inhibit autophagy in Hela cells. In addition, LDM could inhibit the migration and invasion of Hela cells, change the expression levels of EMT-related factors, but LSD1 was not involved in this process. Conclusions LDM can effectively inhibit proliferation, invasion and metastasis of Hela cervical cancer cells, and its mechanism may be closely related to cell cycle arrest, apoptosis, interfering autophagy and inhibition of EMT.