Study on mechanism of action of Blattidae compound PB against chronic ulcerative colitis induced by alloantigen and acetic acid in rats / 中国药理学通报

ABSTRACT

To study the effect of Blattidae compound PB on chronic UC induced by alloantigen combined with acetic acid in rats, and to preliminarily explore its mechanism. Methods Chronic UC model was established using alloantigen combined with acetic acid. Normal group and model group were given sodium chloride solution by enema, while the other groups were given respectively to the SASP, the Changyanning and PB low, medium and high doses. The experiment was determined by evaluating DAI, CMDI, HS and the visceral index. Serum IL-4, IL-8, IL-17, CRP, IgG and colonic mucosa TNF-MPO EGF were detected by ELISA. Results Compared with normal group, the levels of IL-8, IL-17, INF-7, CRP, IgG, TNF-a, MPO in model group significantly increased, while the levels of 1L-4, EGF decreased significantly (P <0. 01, P <0. 05). But the levels of DAI,CMDI, HS, IL-8, IL-17, INF-7, CRP, IgG, TNF-ot, MPO in each dose of PB groups were significantly lower than those of model group, and the levels of IL-4, EGF were significantly higher than those of model group (P <0. 01, P < 0. 05). Conclusions Blattidae compound PB can effectively relieve chronic ulcerative colitis induced by alloantigen combined with acetic acid in rats, and its mechanism may be related to promoting Th2 cell activation, changing Thl-prone Thl/Th2 balance to Th2, which regulated cell immunity and humoral immunity to achieve the steady state immune balance.