Effect of derl1 on migration and invasion of human breast cancer cells zr-75-1 / 中国药理学通报

ABSTRACT

Aim To investigate the effect of DERL1 on migration and invasion of human breast cancer cells ZR-75-1 and its potential mechanism. Methods Plasmid of DERL1 overexpression or DERL1 interference was used to explore the function of DERLI in breast cancer cells ZR-75-1. The migration and inva-sion of ZR-75-1 cells were analyzed using wound healing assays and Transwell assays. In addition, the expression of E-cadherin mRNA and protein in ZR-75-1 cells was detected by Real-time quantitative PCR and immunofluorescence. Results After plasmid of DERLI overexpression transfection for 48 h, the expression of DERLI in ZR-75-1 cells significantly in-creased compared with that of control group ( P < 0.01) , and the migration and invasion of cells were significantly up-regulated ( P < 0. 05 ) , cell morphology was transformed from cobblestone-like epithelial to spindle-shaped mesenchymal, and the expression of E-cadherin mRNA and protein significantly decreased (P <0.05). After plasmid of DERLI interference transfection for 48 h, compared with control group, the ex-pression of DERLI in ZR-75-1 cells was significantly down-regulated ( P < 0. 01 ) , DERLI knockdown inhibited the migration and invasion of ZR-75-1 cells (P < 0.05 ) , cell morphology changed from spindle-shaped mesenchymal to cobblestone-like epithelial, and the expression of E-cadherin mRNA and protein was significantly up-regulated ( P < 0. 05 ). Conclusions DERLI can promote the migration and invasion of human breast cancer cells ZR-75-1, which may be related to its inhibition of E-cadherin expression to promotion of epithelial mesenchymal transition.